R&D services

Some of the common assays that we routinely carry out in our facility to provide an additional resource to researchers and educators are as follows:

Cytotoxicity Assays

  • i. SRB assay ii. LDH assay
  • iii. MTT/XTT/MTS assay
  • iv. Cytolysis assay
  • v. Cell viability
  • vi. Clonogenic assay


    • Cell death and apoptosis markers

  • p53 assay
  • p21 assay
  • γH2AX assay
  • MDM2 assay
  • Topoisomerase I assay
  • Topoisomerase II assay
  • Histone H3 acetylation assay
  • Caspase assays (Caspases-1/3/5/7/8/9)
  • Annexin V/PI
  • Mitochondrial depolarization assay
  • Cytochrome c release assay
  • Cell cycle
  • DNA damage analysis
  • Cyclin-dependent kinases (CDKs) analysis
  • BAX assay
  • Phospho-AKT (p-AKT) assay
  • Bcl-2 assay
  • PARP1 enzyme activity assay
  • NF-kB/p65 assay
  • TUNEL assay
  • Ca2+ assay
  • cAMP assay
  • Akt assay
  • MAPK/ p38 activity assay
  • JNK activity assay
  • c-Jun test
  • HIF-1 alpha assay
  • EGFR assay


    • Cyto-Protective Studies

  • STAT 1/2/3/6 assay
  • iNOS activity assay
  • COX activity assay
  • Nur77 assay
  • B-Cell Linker (BLNK) protein assay
  • NFAT assay
  • NF-kB/p65 assay
  • ATF-2 assay
  • Cytokines or interleukins (IFN, TNF, TGF, IL1/2/6/8/10 etc)
  • Tube formation
  • Cell migration assays- scratch
  • Cell migration assays- cell insert based
  • Angiogenesis signalling pathways (H1F1α, SMAD, p38, E-cadherin, PI3K, etc.)

    • Cellular stress-based Assays

  • ROS formation assay
  • Lipid peroxidation assay
  • Hypoxia signaling marker analysis
  • Glutathione depletion assay
  • Anti-oxidant assays: SOD1, SOD2, catalase (CAT), GSH, GPX and GSR
  • NAD(P)H assay
  • FAD assay
  • LC3B autophagy assay
  • ATP content assay

    • Anti-Diabetic assays

  • Glucose uptake assay
  • α-amylase inhibition assay
  • α-glucosidase inhibition assay
  • Sucrase inhibition assay
  • cAMP assay
  • IGFR1 expression
  • GLPR1 expression
  • IR a/b expression

    • Stem Cell based assays

  • Isolation of MSCs, culture, maintenance (5 x 10^6 cells) and surface markers (6 markers) studies
  • 2D Cytotoxicity assays
  • Differentiation studies (0, 7, 14, 21 days)
  • Differentiation marker studies (0, 7, 14, 21 days)
  • 3D organoid-based cytotoxicity

    • FACS based studies

  • P-glycoprotein (P-gp) activity assay
  • Cellular characterization using cell surface markers
  • Surface and intracellular markers analysis
  • Stem cell markers analysis viz., CD markers etc.
  • CD3/CD4/CD8
  • HLAB27
  • Detection and quantification of circulating Tumor or endothelial cells Plant genome/ ploidy analysis by Flow cytometry

    • ELISA based studies

  • Endotoxin level detection tests
  • Protein quantification
  • Marker based

    • Microbiology Services:

  • Identification of bacteria from environmental and clinical isolates
  • Characterization of bacteria based on the phenotype
  • Assessment to identify microbes that produce antibiotics
  • Investigation of food-borne pathogens
  • Antibiogram studies
  • Invitro studies of standardization and inhibition of biofilm formation
  • Assessment of Bacteriophage activity in a sample
  • Growth curve assay
  • Sterility tests for medicinal products
  • Quality control of water to prevent
  • bacterial growth Anti-quorum sensing studies by screening
  • Preservation of culture bank

    • We also perform various other assays and CRO assignments based on client requirements.

    For any queries, please feel free to write to us or call us. We would be very happy to help you.