R&D services

Some of the common assays that we routinely carry out in our facility to provide an additional resource to researchers and educators are as follows:

Cytotoxicity Assays

  • i. SRB assay ii. LDH assay
  • iii. MTT/XTT/MTS assay
  • iv. Cytolysis assay
  • v. Cell viability
  • vi. Clonogenic assay


    • Cell death and apoptosis markers

  • p53 assay
  • p21 assay
  • γH2AX assay
  • MDM2 assay
  • Topoisomerase I assay
  • Topoisomerase II assay
  • Histone H3 acetylation assay
  • Caspase assays (Caspases-1/3/5/7/8/9)
  • Annexin V/PI
  • Mitochondrial depolarization assay
  • Cytochrome c release assay
  • Cell cycle
  • DNA damage analysis
  • Cyclin-dependent kinases (CDKs) analysis
  • BAX assay
  • Phospho-AKT (p-AKT) assay
  • Bcl-2 assay
  • PARP1 enzyme activity assay
  • NF-kB/p65 assay
  • TUNEL assay
  • Ca2+ assay
  • cAMP assay
  • Akt assay
  • MAPK/ p38 activity assay
  • JNK activity assay
  • c-Jun test
  • HIF-1 alpha assay
  • EGFR assay


    • Cyto-Protective Studies

  • STAT 1/2/3/6 assay
  • iNOS activity assay
  • COX activity assay
  • Nur77 assay
  • B-Cell Linker (BLNK) protein assay
  • NFAT assay
  • NF-kB/p65 assay
  • ATF-2 assay
  • Cytokines or interleukins (IFN, TNF, TGF, IL1/2/6/8/10 etc)
  • Tube formation
  • Cell migration assays- scratch
  • Cell migration assays- cell insert based
  • Angiogenesis signalling pathways (H1F1α, SMAD, p38, E-cadherin, PI3K, etc.)

    • Cellular stress-based Assays

  • ROS formation assay
  • Lipid peroxidation assay
  • Hypoxia signaling marker analysis
  • Glutathione depletion assay
  • Anti-oxidant assays: SOD1, SOD2, catalase (CAT), GSH, GPX and GSR
  • NAD(P)H assay
  • FAD assay
  • LC3B autophagy assay
  • ATP content assay

    • Anti-Diabetic assays

  • Glucose uptake assay
  • α-amylase inhibition assay
  • α-glucosidase inhibition assay
  • Sucrase inhibition assay
  • cAMP assay
  • IGFR1 expression
  • GLPR1 expression
  • IR a/b expression

    • Stem Cell based assays

  • Isolation of MSCs, culture, maintenance (5 x 10^6 cells) and surface markers (6 markers) studies
  • 2D Cytotoxicity assays
  • Differentiation studies (0, 7, 14, 21 days)
  • Differentiation marker studies (0, 7, 14, 21 days)
  • 3D organoid-based cytotoxicity

    • FACS based studies

  • P-glycoprotein (P-gp) activity assay
  • Cellular characterization using cell surface markers
  • Surface and intracellular markers analysis
  • Stem cell markers analysis viz., CD markers etc.
  • CD3/CD4/CD8
  • HLAB27
  • Detection and quantification of circulating Tumor or endothelial cells Plant genome/ ploidy analysis by Flow cytometry

    • ELISA based studies

  • Endotoxin level detection tests
  • Protein quantification
  • Marker based

    • Microbiology Services:

  • Identification of bacteria from environmental and clinical isolates
  • Characterization of bacteria based on the phenotype
  • Assessment to identify microbes that produce antibiotics
  • Investigation of food-borne pathogens
  • Antibiogram studies
  • Invitro studies of standardization and inhibition of biofilm formation
  • Assessment of Bacteriophage activity in a sample
  • Growth curve assay
  • Sterility tests for medicinal products
  • Quality control of water to prevent
  • bacterial growth Anti-quorum sensing studies by screening
  • Preservation of culture bank

    • We also perform various other assays and CRO assignments based on client requirements.

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