R&D services

Some of the common assays that we routinely carry out in our facility to provide an additional resource to researchers and educators are as follows:

Cytotoxicity Assays

  • i. SRB assay ii. LDH assay
  • iii. MTT/XTT/MTS assay
  • iv. Cytolysis assay
  • v. Cell viability
  • vi. Clonogenic assay
  • Cell death and apoptosis markers

  • i.p53 assay
  • ii.p21 assay
  • iii.γH2AX assay
  • iv.MDM2 assay
  • v.Topoisomerase I assay
  • vi.Topoisomerase II assay
  • vii.Histone H3 acetylation assay
  • viii.Caspase assays (Caspases-1/3/5/7/8/9)
  • ix.Annexin V/PI
  • x.Mitochondrial depolarization assay
  • xi.Cytochrome c release assay
  • xii.Cell cycle
  • xiii.DNA damage analysis
  • xiv.Cyclin-dependent kinases (CDKs) analysis
  • xv.BAX assay
  • xvi.Phospho-AKT (p-AKT) assay
  • xvii.Bcl-2 assay
  • xviii.PARP1 enzyme activity assay
  • xix.NF-kB/p65 assay
  • xx.TUNEL assay
  • xxi.Ca2+ assay
  • xxii.cAMP assay
  • xxiii.Akt assay
  • xxiv.MAPK/ p38 activity assay
  • xxv.JNK activity assay
  • xxvi.c-Jun test
  • xxvii.HIF-1 alpha assay
  • xxviii.EGFR assay
  • Cyto-Protective Studies

  • i.STAT 1/2/3/6 assay
  • ii.iNOS activity assay
  • iii.COX activity assay
  • iv.Nur77 assay
  • v.B-Cell Linker (BLNK) protein assay
  • vi.NFAT assay
  • vii.NF-kB/p65 assay
  • viii.ATF-2 assay
  • ix.Cytokines or interleukins (IFN, TNF, TGF, IL1/2/6/8/10 ETC)
  • x.Tube formation
  • xi.Cell migration assays- scratch
  • xii.Cell migration assays- cell insert based
  • xiii.Angiogenesis signalling pathways (H1F1α, SMAD, p38, E-cadherin, PI3K, etc.)
  • Cellular stress-based Assays

  • i.ROS formation assay
  • ii.Lipid peroxidation assay
  • iii.Hypoxia signaling marker analysis
  • iv.Glutathione depletion assay
  • v.Anti-oxidant assays: SOD1, SOD2, catalase (CAT), GSH, GPX and GSR
  • vi.NAD(P)H assay
  • vii.FAD assay
  • viii.LC3B autophagy assay
  • ix.ATP content assay
  • Anti-Diabetic assays

  • x.Glucose uptake assay
  • xi.α-amylase inhibition assay
  • xii.α-glucosidase inhibition assay
  • xiii.Sucrase inhibition assay
  • xiv.cAMP assay
  • xv.IGFR1 expression
  • xvi.GLPR1 expression
  • xvii.IR a/b expression
  • Stem Cell based assays

  • i.Isolation of MSCs, culture, maintenance (5 x 10^6 cells) and surface markers (6 markers) studies
  • ii.2D Cytotoxicity assays
  • iii.Differentiation studies (0, 7, 14, 21 days)
  • iv.Differentiation marker studies (0, 7, 14, 21 days)
  • v.3D organoid-based cytotoxicity
  • FACS based studies

  • i.P-glycoprotein (P-gp) activity assay
  • ii.Cellular characterization using cell surface markers
  • iii.Surface and intracellular markers analysis
  • iv.Stem cell markers analysis viz., CD markers etc.
  • v.CD3/CD4/CD8
  • vi.HLAB27
  • vii.Detection and quantification of circulating Tumor or endothelial cells viii.Plant genome/ ploidy analysis by Flow cytometry
  • ELISA based studies

  • i.Endotoxin level detection tests
  • ii.Protein quantification
  • Microbiology Services:

  • i.Identification of bacteria from environmental and clinical isolates
  • ii.Characterization of bacteria based on the phenotype
  • iii.Assessment to identify microbes that produce antibiotics
  • iv.Investigation of food-borne pathogens
  • v.Antibiogram studies
  • vi.Invitro studies of standardization and inhibition of biofilm formation
  • vii.Assessment of Bacteriophage activity in a sample
  • viii.Growth curve assay
  • ix.Sterility tests for medicinal products
  • x.Quality control of water to prevent
  • bacterial growth xi.Anti-quorum sensing studies by screening
  • xii.Preservation of culture bank
  • We also perform various other assays and CRO assignments based on our client requirements.